- ￥2808 - 7956
- Biorbyt
- orb1238288
- 英国
- 2026年07月31日
- ELISA
- Mouse
- Human
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- 文献和实验
- 技术资料
- 抗体名：
CD274 Antibody (Biotin) 抗体
- 抗体英文名：
CD274 Antibody (Biotin)
- 靶点：
CD274
- 浓度：
1 mg/mL
- 应用范围：
ELISA
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1238288
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Monoclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
IgG1
- 免疫原：
详询
- 规格：
0.02 mg/0.1 mg
| 规格： | 0.02 mg | 产品价格： | ￥2808.0 |
|---|---|---|---|
| 规格： | 0.1 mg | 产品价格： | ￥7956.0 |
产品子类型: Primary Antibody
描述: CD274 Antibody (Biotin)
克隆号: [8E12]
别名: PD-L1 Antibody: Programmed cell death 1 ligand-1, programmed death ligand 1, PDL1, PDL-1, B7-H1
应用说明: PD-L1 antibody can be used for as detection antibody in Sandwich Elisa at 1 μg/mL.
免疫原: PD-L1 antibody was raised against the extracellular domain of human PD-L1.
防腐剂: PD-L1 Antibody is supplied in PBS containing 1% rAlbumin and 0.02% sodium azide.
纯化方法: PD-L1 Antibody is supplied as protein A purified IgG1.
研究领域: Immunology & Inflammation
UniProt ID: Q9NZQ7
NCBI 参考序列: NP_054862
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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