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- 文献和实验
- 技术资料
- 抗体名:
IL31 Antibody (Biotin) 抗体
- 抗体英文名:
IL31 Antibody (Biotin)
- 靶点:
IL31
- 浓度:
0.5 mg/mL
- 应用范围:
ELISA, FC, ICC, IHC, IHC-P, IP, WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1251429
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Biotin
- 克隆性:
Monoclonal
- 保存条件:
详询
- 形态:
Liquid
- 亚型:
IgG2b
- 免疫原:
详询
- 规格:
0.1 mg
产品子类型: Primary Antibody
描述: IL31 Antibody (Biotin)
克隆号: [SB118b]
别名: Interleukin-31
应用说明: IL-31 Antibody [SB118b] for use in ELISA assays.
免疫原: E. coli-expressed human IL-31
防腐剂: Supplied in PBS/NaN3
特异性: IL-31
研究领域: Immunology & Inflammation
UniProt ID: Q6EBC2
NCBI 参考序列: Q6EBC2
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.

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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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