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- 文献和实验
- 技术资料
- 抗体名:
C4A, C4B Antibody 抗体
- 抗体英文名:
C4A, C4B Antibody
- 靶点:
C4A, C4B
- 浓度:
0.2 mg/mL
- 应用范围:
ELISA, IF, IHC
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1251876
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 形态:
Liquid
- 亚型:
IgG1, kappa
- 免疫原:
详询
- 规格:
100 ug
产品子类型: Primary Antibody
描述: C4A, C4B Antibody
克隆号: SPM545
别名: Complement C4-A, Acidic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 2, Complement C4 beta chain, Complement C4-A alpha chain, C4a anaphylatoxin, C4b-A, C4d-A, Complement C4 gamma chain, C4A, CO4, CPAMD2
应用说明: ELISA: 1-2 µg/mL (order BSA/azide free format)Immunofluorescence: 1-2 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the Complement 4d antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: Recombinant human C4d protein was used as the immunogen for this Complement 4d antibody.
防腐剂: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
研究领域: Neuroscience
UniProt ID: P0C0L5, P0C0L4
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.

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文献和实验补体成分 C4(C4a/C4b) 补体成分:C4 血清浓度(μg/ml):200~500 分子量(kDa):210 亚单位(链)及分子量(kDa):α:90β:78γ:33 激活产物:C4a;C4b 生物学活性:C4a 弱的过敏毒素作用 C4b 组成CP中的C3、C5转化
Human Complement Components C4A and C4B Genetic Diversities: Complex Genotypes and Phenotypes
methods that can accurately determine the genotypes and phenotypes of human complement components C4A and C4B. Specifically, they allow investigators to determine how many C4 genes are present in a diploid genome of a human subject and to quantify
Evaluation of Complement-Dependent Cytotoxicity Using ATP Measurement and C1q/C4b Binding
method to evaluate the binding of C1q on the Fc region and the binding of C4b on cell surface. The luminescence method coupled with complement protein analysis by flow cytometry encompasses all needed methods to evaluate antibody ability to trigger CDC.
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