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- 文献和实验
- 技术资料
- 抗体名:
IgM Antibody [SPM188] 抗体
- 抗体英文名:
IgM Antibody [SPM188]
- 浓度:
0.2 mg/mL
- 应用范围:
FC, IF, IHC-P
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1252511
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 形态:
Liquid
- 亚型:
IgG1, kappa
- 免疫原:
详询
- 规格:
100 ug
产品子类型: Primary Antibody
描述: IgM Antibody [SPM188]
克隆号: SPM188
应用说明: Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 0.5-1 µg/mLImmunohistochemistry (FFPE): 0.5-1 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Anti-IgM antibody should be determined by the researcher.1. Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: Heavy chain of human IgM was used as the immunogen for the Anti-IgM antibody.
防腐剂: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
研究领域: Immunology & Inflammation
UniProt ID: P20769, P01871
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.

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文献和实验Anticardiolipin Antibody and Anti-beta 2 Glycoprotein I Antibody Assays
criteria must be met. Laboratory testing to identify aPL antibodies includes lupus anticoagulant (liquid-based clotting assays) and immunological solid-phase assays (usually enzyme-linked immunosorbent assay formats) for IgG and/or IgM anticardiolipin (aCL
。此时若再次接触抗原,可使已消失的抗体快速上升。如再次刺激机体的抗原与初次相同,则称为特异性回忆反应;若与初次反应不同,则称为非特异性回忆反应。非特异性回忆反应引起的抗体的上升是暂时性的,短时间内即很快下降。 抗体的分类 (1)按作用对象,可将其分为抗毒素、抗菌抗体、抗病素抗体和亲细胞抗体(能与细胞结合的免疫球蛋白,如1型变态反应中的lgE反应素抗体,能吸附在靶细胞膜上)。(2)按理化性质和生物学功能,可将其分为IgG、IgA、IgM、IgE、IgD五类。(3)按与抗原结合后是否出现
Protein A Purification of Antibody
PBS, overnight, 2-8℃.Re-equilibrate column with Binding Buffer. Store column at 2-8℃.Determine protein concentration of Ab, aliquot, and store at -20℃.3. NoteExtinction Coefficient for IgG = 1.4 and for IgM = 1.2
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