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- ￥13338
- Biorbyt
- orb1252670
- 英国
- 2026年07月31日
- FC, IF, IHC
- Mouse
- All
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- 文献和实验
- 技术资料
- 抗体名：
BrdU Antibody [BRD494] 抗体
- 抗体英文名：
BrdU Antibody [BRD494]
- 浓度：
0.2 mg/mL
- 应用范围：
FC, IF, IHC
- 宿主：
Mouse
- 适应物种：
All
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb1252670
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 形态：
Liquid
- 亚型：
IgG1
- 免疫原：
详询
- 规格：
100 ug
产品子类型: Primary Antibody
描述: BrdU Antibody [BRD494]
克隆号: BRD494
应用说明: Flow Cytometry: 0.5-1 ug/million cellsIF: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bromodeoxyuridine / BrdU antibody to be titered up or down for optimal performance.1. For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: BrdU / Bromodeoxyuridine conjugated to KLH was used as the immunogen for this antibody.
防腐剂: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
研究领域: Cell Biology
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
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文献和实验a variety of cellular sources from human olfactory epithelium (Hahn et al., 2005) and neural progenitors (Nieoullon et al., 2005) to neural stem cells (Chu et al., 2004). BrdU antibody from Abcam Identification of the labelled cells can be applied
Immunohistochemistry and Detection of Proliferating Cells by BrdU
Immunohistochemistry is a classic technique used for the localization of antigenic target molecules in �tissue. The method exploits the principle that the target antigen is recognized by specific antibody and is visualized using different
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
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