TP53 Antibody 抗体，orb1252690，Bi

类别: Antibodies

产品子类型: Primary Antibody

描述: TP53 Antibody

克隆号: BP53-12

别名: Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53

应用说明: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLWB: 0.5-1 µg/mLIHC (FFPE): 0.5-1 µg/mL for 30 minutes at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

免疫原: Recombinant human wild-type p53 protein was used as the immunogen for this antibody.

防腐剂: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

纯化方法: Protein G affinity chromatography

特异性: Does not react with mouse and rat

研究领域: Cancer Biology, Cell Biology, Signal Transduction

UniProt ID: P04637

保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

备注: For research use only.