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细胞污染识别与处理全攻略:5 种常见类型+关键误区abinScience 体内级抗体,年度锁价,低至 1200五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
EPCAM Antibody 抗体
- 抗体英文名:
EPCAM Antibody
- 靶点:
EPCAM
- 浓度:
0.2 mg/mL
- 应用范围:
FC, IF, IHC
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb1252732
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
详询
- 形态:
Liquid
- 亚型:
IgG1, kappa
- 免疫原:
详询
- 规格:
100 ug
产品子类型: Primary Antibody
描述: EPCAM Antibody
克隆号: 323/A3
别名: Epithelial cell adhesion molecule, Ep-CAM, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, EGP, Epithelial glycoprotein 314, EGP314, hEGP314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, CD326, EPCAM, GA733-2, M1S2, M4S1, MIC18, TACSTD1, TROP1
应用说明: Flow Cytometry: 0.5-1 ug/million cellsIF: 1-2 µg/mLIHC (FFPE): 0.5-1.0 µg/mL for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin/paraffin tissues REQUIRES digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: MCF-7 human breast cancer cells were used as the immunogen for this EpCAM antibody.
防腐剂: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
特异性: Does not react with rat
研究领域: Immunology & Inflammation
UniProt ID: P16422
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.


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文献和实验STM:广州医科大学刘铭/谢茂彬合作发现肝细胞癌的新潜在治疗靶点 Claudin 6
显示,CLDN6 可以稳定 YAP1,促进其核转位,从而活化 Hippo 通路。 IP 结果显示 CLDN6 与 YAP1 竞争性结合 TJP2 鉴于 CLDN6 是多次跨膜蛋白,但其不具有(或尚未发现?)配体结合结构域,难以设计小分子药物;而如果使用单抗的话,由于肝细胞癌具有复杂的微环境, T 细胞难以发挥足够的抗体依赖细胞毒性(ADCC)效应杀伤癌细胞。因此,作者设计了靶向 CLDN6 的抗体偶联药物(Antibody-Drug Conjugate, ADC),将细胞毒性药物 DM1 和 CLDN6 抗体
grafts from the same patient (graft). Stains shown are hematoxylin and eosin (H&E) as well as antibody stains for ER, PR, HER2, cytokeratin (CK), E‐cadherin (E‐cad), β‐catenin (β‐cat), and human‐specific vimentin (hVim). Positive antibody signals
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