- ￥4329 - 8775
- Biorbyt
- orb749791
- 英国
- 2026年07月29日
- FACS, IF, IHC-P, WB
- Mouse
- Human
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- 技术资料
- 抗体名：
Fascin Antibody 抗体
- 抗体英文名：
Fascin Antibody
- 应用范围：
FACS, IF, IHC-P, WB
- 宿主：
Mouse
- 适应物种：
Human
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb749791
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG2a, kappa
- 免疫原：
详询
- 规格：
20 ug/100 ug
| 规格： | 20 ug | 产品价格： | ￥4329.0 |
|---|---|---|---|
| 规格： | 100 ug | 产品价格： | ￥8775.0 |
类别: Antibodies
产品子类型: Primary Antibody
描述: Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. [UniProt]
克隆号: SPM133
应用稀释比例: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
应用说明: Optimal dilution of the Fascin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: Full length recombinant human protein was used as the immunogen for the Fascin antibody.
防腐剂: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
研究领域: Musculoskeletal & Connective Tissue Research
UniProt ID: Q16658
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: Fascin organizes filamentous actin into bundles with a minimum of 4.1:1 actin/fascin ratio. Plays a role in the organization of actin filament bundles and the formation of microspikes, membrane ruffles, and stress fibers. Important for the formation of a diverse set of cell protrusions, such as filopodia, and for cell motility and migration. [UniProt]
克隆号: SPM133
应用稀释比例: Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
应用说明: Optimal dilution of the Fascin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
免疫原: Full length recombinant human protein was used as the immunogen for the Fascin antibody.
防腐剂: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
纯化方法: Protein G affinity chromatography
研究领域: Musculoskeletal & Connective Tissue Research
UniProt ID: Q16658
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
IHC analysis of formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with Fascin antibody (clone SPM133).
Immunofluorescent staining of permeabilized human HeLa cells with Fascin antibody (clone SPM133, green), Phalloidin (actin, red) and Hoescht (nucleus, blue).
Flow cytometry testing of PFA-fixed human K562 cells with Fascin antibody (clone SPM133); Red = isotype control, Blue = Fascin antibody.
Western blot testing of Fascin antibody and human HeLa cell lysate (clone SPM133). Predicted molecular weight ~55 kDa.
SDS-PAGE analysis of purified, BSA-free Fascin antibody (clone SPM133) as confirmation of integrity and purity.
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Fascin Antibody 抗体，orb749791，Biorbyt
￥4329 - 8775
