Nucleoli Marker Antibody / Hum

类别: Antibodies

产品子类型: Primary Antibody

描述: This mAb is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone NM95 antibody recognizes an antigenic marker associated with the nucleoli in human cells. The antibody can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells. The nucleoli marker antibody can be used with Fixative fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

克隆号: NM95

应用稀释比例: Flow cytometry: 1-2ug/10^6 cells ,Immunofluorescence: 1-2ug/ml,Immunocytochemistry: 1-2ug/ml for 30 min at RT,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

应用说明: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nucleoli marker antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

免疫原: Nuclei of myeloid leukemia biopsy cells were used as the immunogen.

防腐剂: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

纯化方法: Protein G affinity chromatography

研究领域: Immunology & Inflammation

保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

备注: For research use only.