Basic Cytokeratin Antibody 抗体，

类别: Antibodies

产品子类型: Primary Antibody

描述: There are two types of cytokeratins/keratins/CKs: the acidic type I cytokeratins and the basic or neutral type II cytokeratins. The subsets of cytokeratins which an epithelial cell expresses depends mainly on the type of epithelium, the moment in the course of terminal differentiation and the stage of development. Thus this specific keratin fingerprint allows the classification of all epithelia upon their keratin expression profile. Furthermore this applies also to the malignant counterparts of the epithelia (carcinomas), as the keratin profile tends to remain constant when an epithelium undergoes malignant transformation. The main clinical implication is that the study of the keratin profile by immunohistochemistry techniques is a tool of immense value widely used for tumor diagnosis and characterization in surgical pathology. [Wiki]

克隆号: BCCK1-1

应用稀释比例: Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT (1),Prediluted IHC only format: incubate for 30 min at RT (2)

应用说明: Titering of the Basic Cytokeratin antibody may be required for optimal performance.1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.3. This Basic Cytokeratin antibody detects the following keratins/CKs: CK1 (67kDa), CK3 (64kDa), CK4 (59kDa), CK5 (58 kDa), CK6 (56kDa), CK8 (52 kDa).

免疫原: An amino acid sequence common to basic keratins was used as the immunogen for the Basic Cytokeratin antibody.

防腐剂: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide

纯化方法: Protein G affinity chromatography

研究领域: Neuroscience

UniProt ID: Q01546

保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

备注: For research use only.