Rab5 Antibody (APC) 抗体，orb1518

类别: Antibodies

描述: Rabbit polyclonal to Rab5 (APC). Rab5 is a 24kDa member of the Rab family of small guanosine triphosphatases (GTPases), Ras superfamily. Rab GTPases are central regulators of membrane trafficking in the eukaryotic cell. Their regulatory capacity depends on their ability to cycle between the GDP -bound inactive and GTP-bound active states. This conversion is regulated by GDP/GTP exchange factors (GEPs), GDP dissociation inhibitors (GDIs) and GTPase-activating proteins (GAPs). Activation of a Rab protein is coupled to its association with intracellular membranes, allowing it to recruit downstream effector proteins to the cytoplasmic surface of a subcellular compartment. Through these proteins, Rab GTPases regulate vesicle formation, actin- and tubulin-dependent vesicle movement, and membrane fusion(1). Rab proteins contain conserved regions involved in guanine-nucleotide binding, and hyper variable COHO-terminal domains with a cysteine motif implicated in subcellular targeting. Post-translational modification of the cysteine motif with one or two geranyl groups is essential for the membrane association and correct intracellular localization of Rab proteins(3). Each Rab shows a characteristic subcellular distribution. In particular, Rab5 is ubiquitously expressed in human tissues. It localizes mainly to early endosomes, but is also present on the plasma membrane. It regulates the fusion between endocytic vesicles and early endosomes, as well as the homotypic fusion between early endosomes. Among the proteins recruited by the GTP-bound active Rab5 are Rabaptin-5 and EEA1. Anti-Rab5 may be used as an early endosome marker....

别名: Rab 5A, RAS associated protein RAB5A, Ras related protein Rab 5 A

应用稀释比例: WB (1:1000), IHC (1:100), ICC/IF (1:80)

应用说明: 1 µl/ml was sufficient for detection of Rab5 in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

免疫原: Human Rab5 synthetic peptide conjugated to KLH; identical to dog Rab5 sequence over the residues

分子量: 26kDa

防腐剂: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA

纯化方法: Protein A Purified

特异性: Detects ~26kDa.

研究领域: Signal Transduction

UniProt ID: P20339

NCBI 参考序列: NP_004153.2

Entrez 基因 ID: 5868

保存条件: Conjugated antibodies should be stored according to the product label

备注: For research use only.