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- 文献和实验
- 技术资料
- 抗体名:
Ubiquitin Antibody (FITC) 抗体
- 抗体英文名:
Ubiquitin Antibody (FITC)
- 靶点:
Ubiquitin
- 浓度:
1 mg/ml
- 应用范围:
ICC, IF, IHC, IP, WB
- 宿主:
Rabbit
- 适应物种:
Bovine, Canine, Drosophila, Fish, Frog, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Yeast
- 保质期:
12 months from date of receipt.
- 抗原来源:
详询
- 目录编号:
orb151480
- 级别:
科研
- 库存:
99
- 供应商:
Biorbyt
- 标记物:
FITC
- 克隆性:
Polyclonal
- 保存条件:
详询
- 免疫原:
详询
- 规格:
200 ul
描述: Rabbit polyclonal to Ubiquitin (FITC). Ubiquitin is a small protein that occurs in all eukaryotic Ubiquitin is a small protein that occurs in all eukaryotic...
别名: Polyubiquitin B, RPS27A, UBA52, UBB, UBC, ubiquitin B
应用稀释比例: WB (1:1000), IHC (1:100), ICC/IF (1:100)
应用说明: A 1:1000 dilution was sufficient for detection of free ubiquitin in 15 µg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.
免疫原: Native bovine Ubiquitin, conjugated to KLH
分子量: 10kDa
防腐剂: 640.91mM DMSO, 136.36 mM Ethanolamine, 126.89 mM chlorides, 9.09mM phosphates, 9.09mM NaHCO3
纯化方法: Peptide Affinity Purified
特异性: Detects ~10kDa. It also recognizes ubiquinated proteins.
研究领域: Neuroscience, Protein Biochemistry, Signal Transduction
UniProt ID: P0CG53
NCBI 参考序列: NP_776558.1
Entrez 基因 ID: 281370
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.





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文献和实验Studies of the Ubiquitin Proteasome System
) 6% to 8% SDS‐PAGE minigels (unit 6.1 ) Anti‐ubiquitin antibody ( protocol 31 ; for immunoblot detection) Boiling water bath Additional reagents and equipment for SDS
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
8.0) in clean 16x125mm polypropylene test tube. If needed, adjust pH to 8.0 using 0.5M carbonate buffer. 2. Prepare fresh, 1mg/mL of either NHS-FITC in DMSO or 1mg/mL NHS-LC-Biotin in DH2O. If FITC coupling, add 75uL of FITC/DMSO solution to Antibody
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