- ￥8658
- Biorbyt
- orb396579
- 英国
- 2026年07月24日
- ELISA, FACS, FC, ICC, IF, WB
- Mouse
- All
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- 文献和实验
- 技术资料
- 抗体名：
4-Hydroxy-2-hexenal Antibody (Biotin) 抗体
- 抗体英文名：
4-Hydroxy-2-hexenal Antibody (Biotin)
- 靶点：
4-Hydroxy-2-hexenal
- 浓度：
1 mg/ml
- 应用范围：
ELISA, FACS, FC, ICC, IF, WB
- 宿主：
Mouse
- 适应物种：
All
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb396579
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
IgG2b
- 免疫原：
详询
- 规格：
100 ug
描述: Mouse monoclonal antibody against 4-Hydroxy-2-hexenal conjugated to Biotin.
克隆号: 6F10
别名: 4-Hydroxy-2-hexenal, 4-Hydroxyhex-2-enal, 4-Hydroxyhexenal, 4-Hydroxy Hexenal, Hex-2-enal, 4-hydroxy-, Hexenal, 4-hydroxy-, 2-Hexenal, 4-hydroxy-, (E)-, (2E)-4-Hydroxy-2-hexenal, (E)-4-Hydroxy-2-hexenal
应用稀释比例: WB (1:1000); ICC/IF (1:50); ELISA (1:1000); FACS (1:50); FCM (1:50)
应用说明: A 1:1000 dilution was sufficient for detection of 4-Hydroxy-2-hexenal in 0.5 µg of 4-Hydroxy-2-hexenal conjugated to BSA and 4-Hydroxy nonenal conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody.
免疫原: Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).
防腐剂: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate
纯化方法: Protein G Purified
特异性: Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.
研究领域: Cancer Biology, Cell Biology, Metabolism Research
保存条件: Conjugated antibodies should be stored according to the product label
备注: For research use only.
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文献和实验4-Hydroxy-2-Nonenal (4-HNE) Staining by Anti-HNE Antibody
using an anti-4-HNE monoclonal antibody (MAb) (6 –8 ) and labeled goat anti-mouse IgG antibody (9 ).
The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
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