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- 文献和实验
- 技术资料
- 亚型:
IgG2b/Kappa
- 保存条件:
零下20度保存,避免反复冻融
- 克隆性:
鼠单抗
- 保质期:
12个月
- 供应商:
景杰生物
- 宿主:
小鼠
- 应用范围:
[ICC/IF,人],[FC,人]
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文献和实验T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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