- ￥4329
- Biorbyt
- orb44036
- 英国
- 2026年07月23日
- FC, IHC-P, IP, WB
- Human, Primate
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- 文献和实验
- 技术资料
- 抗体名：
CD55 Antibody (Biotin) 抗体
- 抗体英文名：
CD55 Antibody (Biotin)
- 靶点：
CD55
- 浓度：
1 mg/ml
- 应用范围：
FC, IHC-P, IP, WB
- 适应物种：
Human, Primate
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb44036
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Biotin
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgM
- 免疫原：
详询
- 规格：
0.1 mg
产品子类型: Primary Antibody
描述: Mouse Monoclonal to CD55.
克隆号: MEM-118
别名: DAF, CROM, CHAPLE
应用说明: Flow cytometry: Recommended dilution: 5-8 μg/ml.
免疫原: HPB-ALL human T cell line
防腐剂: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
纯化方法: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
RRID: AB_10997822
特异性: The antibody MEM-118 recognizes an epitope in SCR4 domain of CD55 (Decay accelerating factor, DAF), a 60-70 kDa glycosylphosphatidylinositol (GPI)-anchored single chain extracellular glycoprotein. CD55 is widely expressed on hematopoietic and on many non-hematopoietic cells; it is weakly present on NK cells.
研究领域: Immunology & Inflammation
UniProt ID: P08174
Entrez 基因 ID: 1604
保存条件: Store at 2-8°C. Do not freeze.
备注: For research use only.
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文献和实验The Avidin-Biotin Complex (ABC) Method and Other Avidin-Biotin Binding Methods
These methods involve, at their core, the vitamin biotin and the protein avidin, which bind together irreversibly. By establishing a biotin link, through avidin, between the horseradish peroxidase enzyme and a secondary antibody reagent
The Avidin-Biotin Complex(ABC) Method
, between the horseradish peroxidase enzyme and a secondary antibody reagent, enzyme localization can be achieved at the site of primary antibody interaction with the specimen (see ) Fig. 1 ). The biotin molecule is small and can be easily conjugated to immunoglobulin
sample to 1 mg/ml (or less). Prepare 1 mg/ml solution of NHS-Biotin in DMSO. Add NHS-Biotin in DMSO to antibody at desired ratio e.g. for 1:8 ratio add 125 ml of NHS-Biotin to 1mg of Ab Incubate on rotator for 4 hours at room
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