- ￥5499
- Biorbyt
- orb334572
- 英国
- 2026年07月22日
- FC, ICC, IF, IHC, WB
- Rabbit
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
PGP9.5/UCHL1/PGP9 Rabbit Polyclonal Antibody 抗体
- 抗体英文名：
PGP9.5/UCHL1/PGP9 Rabbit Polyclonal Antibody
- 靶点：
Ubiquitin carboxyl-terminal hydrolase isozyme L1
- 浓度：
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- 应用范围：
FC, ICC, IF, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb334572
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
Rabbit IgG
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: PGP9.5/UCHL1/PGP9 Rabbit Polyclonal Antibody
别名: Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; 3.4.19.12; 6.-.-.-; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1
应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of human PGP9.5, different from the related mouse and rat sequences by two amino acids.
交叉反应: No cross-reactivity with other proteins
分子量: 25 kDa
防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cell Biology, Epigenetics & Chromatin
UniProt ID: P09936
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: PGP9.5/UCHL1/PGP9 Rabbit Polyclonal Antibody
别名: Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCH-L1; 3.4.19.12; 6.-.-.-; Neuron cytoplasmic protein 9.5; PGP 9.5; PGP9.5; Ubiquitin thioesterase L1; UCHL1
应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry (Fixed), 1-3μg/1x10^6 cells, Human
免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of human PGP9.5, different from the related mouse and rat sequences by two amino acids.
交叉反应: No cross-reactivity with other proteins
分子量: 25 kDa
防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cell Biology, Epigenetics & Chromatin
UniProt ID: P09936
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
Flow Cytometry analysis of K562 cells using anti-PGP9.5 antibody. Overlay histogram showing K562 cells (Blue line). To facilitate intracellular staining, cells were fixed with 4% Fixative and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PGP9.5 Antibody (1 µg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10 µg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x10^6) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
IF analysis of PGP9.5 using anti-PGP9.5 antibody. PGP9.5 was detected in immunocytochemical section of SH-SY5Y cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2 µg/mL rabbit anti-PGP9.5 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of PGP9.5 using anti-PGP9.5 antibody. PGP9.5 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-PGP9.5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of PGP9.5 using anti-PGP9.5 antibody. PGP9.5 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-PGP9.5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of PGP9.5 using anti-PGP9.5 antibody. PGP9.5 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-PGP9.5 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of PGP9.5 using anti-PGP9.5 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PGP9.5 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PGP9.5 at approximately 25 kDa. The expected band size for PGP9.5 is at 25 kDa.
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PGP9.5/UCHL1/PGP9 Rabbit Polyclonal Antibody 抗体，orb334572，Biorbyt
￥5499
