Osteopontin/SPP1 Rabbit Polyclonal Antibody 抗体,orb308760,Biorbyt产品图

Osteopontin/SPP1 Rabbit Polycl

onal Antibody 抗体,orb308760,Biorbyt
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  • ¥5499
  • Biorbyt已认证
  • orb308760
  • 英国
  • 2026年07月22日
  • ELISA, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Osteopontin/SPP1 Rabbit Polyclonal Antibody 抗体

    • 抗体英文名

      Osteopontin/SPP1 Rabbit Polyclonal Antibody

    • 靶点

      Osteopontin

    • 浓度

      Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

    • 应用范围

      ELISA, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      12 months from date of receipt.

    • 抗原来源

      详询

    • 目录编号

      orb308760

    • 级别

      科研

    • 库存

      99

    • 供应商

      Biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      详询

    • 形态

      Lyophilized

    • 亚型

      Rabbit IgG

    • 免疫原

      详询

    • 规格

      100 ug

    类别: Antibodies

    产品子类型: Primary Antibody

    描述: Anti-Osteopontin/SPP1 Antibody. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.

    别名: Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; SPP1; BNSP, OPN; PSEC0156

    应用稀释比例: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, - ELISA, 0.1-0.5μg/ml

    免疫原: A synthetic peptide corresponding to a sequence at the C-terminus of human Osteopontin, different from the related mouse sequence by eight amino acids, and from the related rat sequence by seven amino acids.

    交叉反应: No cross-reactivity with other proteins

    分子量: 66 kDa, 25-55 kDa

    防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

    纯化方法: Immunogen affinity purified.

    复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

    特异性: No cross reactivity with other proteins.

    研究领域: Cancer Biology, Cardiovascular Research, Cell Biology, Musculoskeletal & Connective Tissue Research, Signal Transduction, Stem Cell & Developmental Biology

    UniProt ID: P10451

    保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    备注: For research use only.

    产品细节图片1
    Western blot analysis of Osteopontin using anti-Osteopontin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human SHG-44 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osteopontin antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Osteopontin at approximately 66, 25-55 KD. The expected band size for Osteopontin is at 35 KD.

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    图标文献和实验
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