Nucleobindin 2/NUCB2 Rabbit Po

lyclonal Antibody 抗体，orb334609，Biorbyt

价格￥5499

品牌Biorbyt
货号orb334609
产地英国
更新日期2026年07月22日

应用范围ELISA, IHC, WB
宿主Rabbit
适应物种Mouse, Rat

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抗体名：
Nucleobindin 2/NUCB2 Rabbit Polyclonal Antibody 抗体
抗体英文名：
Nucleobindin 2/NUCB2 Rabbit Polyclonal Antibody
靶点：
Nucleobindin-2
浓度：
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
应用范围：
ELISA, IHC, WB
宿主：
Rabbit
适应物种：
Mouse, Rat
保质期：
12 months from date of receipt.
抗原来源：
详询
目录编号：
orb334609
级别：
科研
库存：
99
供应商：
Biorbyt
标记物：
Unconjugated
克隆性：
Polyclonal
保存条件：
详询
形态：
Lyophilized
亚型：
Rabbit IgG
免疫原：
详询
规格：
100 ug

类别: Antibodies

产品子类型: Primary Antibody

描述: Nucleobindin 2/NUCB2 Rabbit Polyclonal Antibody

别名: Nucleobindin-2; DNA-binding protein NEFA; Prepronesfatin; Nesfatin-1; Nucb2; Nefa

应用稀释比例: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat ELISA, 0.1-0.5μg/ml, - Western blot, 0.1-0.5μg/ml, Mouse

免疫原: E. coli-derived mouse Nucleobindin 2 recombinant protein (Position: V25-L106). Mouse Nucleobindin 2 shares 85.4% and 97.6% amino acid (aa) sequence identity with human and rat Nucleobindin 2, respectively.

交叉反应: No cross-reactivity with other proteins.

分子量: 55 kDa

防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

纯化方法: Immunogen affinity purified.

复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

特异性: No cross reactivity with other proteins.

研究领域: Cell Biology, Epigenetics & Chromatin, Signal Transduction

UniProt ID: P81117

保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

备注: For research use only.

IHC analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody. Nucleobindin 2 was detected in a paraffin-embedded section of mouse gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Nucleobindin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody. Nucleobindin 2 was detected in a paraffin-embedded section of rat gaster tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Nucleobindin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of Nucleobindin 2 using anti-Nucleobindin 2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HEPA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nucleobindin 2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Nucleobindin 2 at approximately 55 kDa. The expected band size for Nucleobindin 2 is at 50 kDa.

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