- ￥5499
- Biorbyt
- orb251521
- 英国
- 2026年07月22日
- ICC, IHC, WB
- Rabbit
- Human, Mouse, Rat
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- 文献和实验
- 技术资料
- 抗体名：
LYRIC/MTDH Rabbit Polyclonal Antibody 抗体
- 抗体英文名：
LYRIC/MTDH Rabbit Polyclonal Antibody
- 靶点：
Protein LYRIC
- 浓度：
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- 应用范围：
ICC, IHC, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb251521
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
详询
- 形态：
Lyophilized
- 亚型：
Rabbit IgG
- 免疫原：
详询
- 规格：
100 ug
类别: Antibodies
产品子类型: Primary Antibody
描述: LYRIC/MTDH Rabbit Polyclonal Antibody
别名: Protein LYRIC; 3D3/LYRIC; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; MTDH; AEG1, LYRIC
应用稀释比例: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Western blot, 0.1-0.5μg/ml
免疫原: E.coli-derived human LYRIC recombinant protein (Position: D101-Q270). Human LYRIC shares 94% amino acid (aa) sequence identity with both mouse and rat LYRIC.
交叉反应: No cross-reactivity with other proteins
分子量: 70-80 kDa
防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cancer Biology, Cell Biology, Signal Transduction
UniProt ID: Q86UE4
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
产品子类型: Primary Antibody
描述: LYRIC/MTDH Rabbit Polyclonal Antibody
别名: Protein LYRIC; 3D3/LYRIC; Astrocyte elevated gene-1 protein; AEG-1; Lysine-rich CEACAM1 co-isolated protein; Metadherin; Metastasis adhesion protein; MTDH; AEG1, LYRIC
应用稀释比例: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Western blot, 0.1-0.5μg/ml
免疫原: E.coli-derived human LYRIC recombinant protein (Position: D101-Q270). Human LYRIC shares 94% amino acid (aa) sequence identity with both mouse and rat LYRIC.
交叉反应: No cross-reactivity with other proteins
分子量: 70-80 kDa
防腐剂: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
纯化方法: Immunogen affinity purified.
复溶/重构: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
特异性: No cross reactivity with other proteins.
研究领域: Cancer Biology, Cell Biology, Signal Transduction
UniProt ID: Q86UE4
保存条件: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
备注: For research use only.
IHC analysis of LYRIC using anti-LYRIC antibody. LYRIC was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-LYRIC Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of LYRIC using anti-LYRIC antibody. LYRIC was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-LYRIC Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of LYRIC using anti-LYRIC antibody. LYRIC was detected in immunocytochemical section of SMMC-7721 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 1 µg/ml rabbit anti-LYRIC Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of LYRIC using anti-LYRIC antibody. LYRIC was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-LYRIC Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of LYRIC using anti-LYRIC antibody. LYRIC was detected in paraffin-embedded section of Rat Skeletal Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-LYRIC Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Western blot analysis of LYRIC using anti-LYRIC antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: U-87MG whole cell lysates, Lane 2: Hela whole cell lysates, Lane 3: MDA-MB-453 whole cell lysates, Lane 4: PC-3 whole cell lysates, Lane 5: A431 whole cell lysates, Lane 6: CACO-2 whole cell lysates, Lane 7: HepG2 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYRIC antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LYRIC at approximately 70-80 KD. The expected band size for LYRIC is at 70-80 KD.
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LYRIC/MTDH Rabbit Polyclonal Antibody 抗体，orb251521，Biorbyt
￥5499
