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文献和实验Expression of an Integral Membrane Protein, the 5HT5A Receptor
of this group of proteins and how they function within the confines of the cellular membranes. Among the different families of membrane proteins, the so-called G protein-coupled receptors (GPCRs) comprise the largest family. From the viewpoint of pharmacology
Isolation of Total RNA from Difficult Tissues
The often exacting process of isolating intact total RNA from tissue becomes even more difficult when processing certain problematic tissues. Fibrous tissues and tissues rich in protein, DNA and nucleases present distinct
Total Protein Extraction with TCA-Acetone
We describe a procedure allowing extraction of total proteins that performs efficiently with a large variety of plant tissues, based on simultaneous precipitation and denaturation with TCA and 2ME in cold acetone. We also describe protein
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