- 询价
- 北京安必奇
- DPATB-H81057
- 2026年07月14日
- ELISA, ICC
- Rabbit
- Trypanosoma brucei
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- 文献和实验
- 技术资料
- 抗体名：
Anti-Adenylate Kinase 1 Antibody
- 抗体英文名：
Anti-Adenylate Kinase 1 (internal region) polyclonal antibody
- 靶点：
AK1
- 应用范围：
ELISA, ICC
- 宿主：
Rabbit
- 适应物种：
Trypanosoma brucei
- 保质期：
See COA
- 抗原来源：
Synthetic Peptide
- 级别：
RUO
- 库存：
In Stock
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 保存条件：
Store at +4°C short term. Aliquot and store at-20°C long term. Avoid repeated freeze / thaw cycles.
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
Synthetic peptide derived from internal domain of Trypanosoma brucei Adenylate kinase 1 protein.
- 规格：
200 μl
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文献和实验Protocol for anti-HA antibody Western Blotting
film for ~2 mins. Notes on the primary antibody: Source : Covance/Babco Description: Crude ascites fluid monoclonal anti-HA antibody (CATLN#135032002, 11mms-101R, 0.5 ml) Handling: Aliquoted into 10ul portions in -20℃ Internal notes: labelled anti
Protocol for anti-HA antibody Western Blotting
it around for 5-10 seconds, and pour it off: this method was worked out in the Yamamoto lab eons ago). 7) Incubate with secondary antibody (anti-mouse) in 5% BSA/PBS/Tween as you would ordinarily. I usually go for 2 hours @ room temp. 8) 12-15 quick washes
. A rabbit polyclonal antisera is also available (101c500; BAbCO) but in our hands this was less reactive. The antibody cleanup protocol is now a seperate document Protocol Cells are grown in 5 mls of YPAD to an O.D. 600 of 0.75 to 1.
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