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- 文献和实验
- 技术资料
- 抗体名:
Zebrafish antibody
- 抗体英文名:
Anti-Zebrafish monoclonal antibody, clone znp7
- 靶点:
Zebrafish
- 应用范围:
IHC
- 宿主:
Mouse
- 适应物种:
Zebrafish
- 保质期:
See COA
- 抗原来源:
Unknown
- 级别:
RUO
- 库存:
In Stock
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 亚型:
IgG1, κ
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文献和实验Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Early development of primary motor neurons and somites in Zebrafish Embryos
boundaries. The ZNP-1 and F6 treated embryos will be treated with a secondary antibody, Fluorescent Goat anti-mouse IgG, so that the stained neurons and somites can be observed under a fluorescent microscope. Procedure: 1. Dechorionate 24
Whole mount antibody staining of zebrafish embryos for markers of segmentation
, 10 minutes each. 3. Incubate overnight at 4oC (cold room) in 0.5 ml primary antibody in 0.2% saponin in PBS. Primary antibodies: (A) znp-1 @ 1/2000 (primary motoneurons) (B)F6@ 1/500 (somite boundaries) (C) no antibody (Control)
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