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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
Anti-ANPEP MAb
- 抗体英文名:
Anti-ANPEP monoclonal antibody, clone 507DU0.2.2
- 靶点:
ANPEP
- 应用范围:
WB
- 宿主:
Mouse
- 适应物种:
Human
- 保质期:
See COA
- 抗原来源:
Unknown
- 级别:
RUO
- 库存:
In Stock
- 标记物:
Unconjugated
- 克隆性:
Monoclonal
- 保存条件:
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
- 形态:
Mouse monoclonal antibody supplied in crude ascites with 0.09% (W/V) sodium azide.
- 亚型:
IgG1
- 规格:
100 μl
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文献和实验Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Purification Of Anti Peptide Antibody
antibody that could contribute to staining background. The proportion in each pool varies with the peptide immunogen. The quality of the anti-peptide serum seems to increase with multiple boosts - the first bleed may be feeble.
Monoclonal Antibody Production Protocol
- PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase
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