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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at 2-8°C for short term. Store at -20°C for long term
- 保质期:
See COA
- 英文名:
Recombinant M. tuberculosis Double-ESAT6 protein
- 库存:
In Stock
- 规格:
100 μg, 200 μg, 500 μg, 1 mg
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文献和实验Non-fluorescent protein/RNA double-labeling
1. Collect embryos, dechorionate and fix in a (1:1) mixture of heptane:fix. Fix is 5% formaldehyde, 0.05M EGTA in 1X PBS. pH 7.0. Devitellinize embryos by shaking in an equal mixture of methanol:heptane (embryos will sink
Recombinant DNA Engineering, Or Cloning Genes In Plasmids
, and so on. If the DNA molecule has promoter elements (to allow RNA polymerases to transcribe the DNA), one can also obtain usable amounts of pure RNA, and RNA with a coding region can be translated in vitro or in vivo to express a protein of interest. The PCR
Non-fluorescent protein/RNA double-labeling
Non-fluorescent protein/RNA double-labeling (a version of this is described in: Manoukian, A. and Krause, H.M.(1992) Genes Dev ., 6: 1740-1751.) 1. Collect embryos, dechorionate and fix in a (1:1) mixture of heptane:fix. Fix
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