Recombinant N. gonorrhoeae Maj

Regulated Recombinant Protein Production in the Antarctic Bacterium Pseudoalteromonas haloplanktis TAC125

genomic region from Pseudoalteromonas haloplanktis TAC125; in particular, a two-component regulatory system was identified. It is involved in the transcriptional regulation of the gene coding for an outer membrane porin (PSHAb0363) that is strongly induced

Detection of Protein‐Protein Interactions by Coprecipitation

of interest is precipitated, and the precipitate is tested for a second specifically associated protein. The approach can be used for native or epitope?tagged proteins for which antibodies are available, or for recombinant proteins that bind with high affinity to a molecule

Heterologous Expression and Affinity Purification of Eukaryotic Membrane Proteins in View of Functional and Structural Studies: The Example of the Sar

of galactose induction steps and increasing the amount of Gal4p transcription factor both improved expression. Lowering the temperature from 28 to 18�C during expression enhanced the recovery of detergent-extractible active protein. In the “light membrane