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- 文献和实验
- 技术资料
- 保存条件：
-20°C Reagent / 4°C blast
- 英文名：
PE 5 reagent + PE Blast Transfection Reagent, 5-10 L Cell Culture
- 规格：
1 KIT
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文献和实验Extraction of DNA using DNAzol Reagent
of cells and nuclei: 1) Cells grown in monolayer: Add 0.75-1.0 ml of DNAzol® Reagent per 10 cm2 culture plate area. Lyse the cells by agitating the culture plate and gently pipet the lysate into an assay tube. 2) Cell Pellets or Suspensions: Add
Extraction of DNA using DNAzol® Reagent
the culture plate and gently pipet the lysate into an assay tube. Cell Pellets or Suspensions: Add 1 ml of DNAzol® Reagent to 1-3 × 107 cells, either in pellet or in suspension (volume 100 µl), pellet the cells and wash them with 0.9% NaCl. Pellet
Procedure for Transfection of Mammalian Cells
(containing nonessential amino acids). Solution B: For each transfection, dilute 12 μl LIPOFECTAMINE Reagent in 375 μl serum-free IMDM. Combine the two solutions, mix gently, and incubate at room temperature for 15-45 min. The solution may appear
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