- 询价
- 北京安必奇
- PHRC331
- 2026年07月14日
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NH2-PEG-SS-COOH
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文献和实验to shake for an additional 10min. 5. Centrifuge the lysates at 7,000rpm for 10min (in a Sorvall SS34 rotor) to pellet cellular debris. Transfer the supernatant to a fresh tube. 17x100mm disposable polypropylene tubes (Falcon brand has pop-top cap
. This problem can be overcome by using a more dilute phage stock. 4.After the cultures clear, add 100μl chloroform and continue to shake for an additional 10min. 5.Centrifuge the lysates at 7,000rpm for 10min (in a Sorvall SS34 rotor) to pellet cellular
, swirl to mix, and incubate in an ice-water bath for 1-2 hours. 8a. Collect the PEG-precipitated DNA by centrifugation in 250 ml bottles at 7000 rpm for 20 minutes at 4degC in the RC5-B using the GSA rotor. 9a. Dissolve the pellets in a combined
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