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- 2026年07月14日
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- 文献和实验
- 技术资料
- 英文名：
pH sensitive acylhydrazone bonded chitosan HCCS-g-PEI-g-PEG
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文献和实验, 0.2 g of KCl, 1.44 g of Na2 HPO4 , and 0.24 g of KH2 PO4 in 800 ml of distilled H2O. Adjust the pH to 7.4 (or 7.2 if required) with HCl. Add H2O to 1 liter. Dispense the solution into aliquots and sterilize them by autoclaving for 20 minutes at 15 psi
Liquid-Liquid Partition Chromatography (LLPC)
. 4 ). The bottom phase of the two-phase system is adsorbed onto a support and packed into a column, which is eluted with the corresponding top phase Fig. 1 ). The LLPC method is a rapid (≤ 2 h), sensitive (0.1-100 �g protein is required) analytical method
was tested on a non-transformed rat fibroblast cell line (Rat1A) which is less sensitive to apoptotic stimuli. Rat1A cells were transiently transfected using either LipofectAMINE as described for the [beta] tumor cells above, or polyethylenimine-(PEI 2000
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