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文献和实验DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
greenish-blue, but increases background, making the staining more equivocal. Other modified indolyl-based compounds can be used if alternative colored precipitates are desired. Salmon-gal (Biosynth International) results in a light orange/pink precipitate
, all of which have galactose linked through a ß-D-glycosidic linkage to a moiety whose properties change upon liberation from galactose (Wallenfels and Weil, 1972). Several substrates yield colored or fluorescent soluble products which are useful when quantifying ß-gal
Protocol for evaluating vascular permeability in wildtype
/kg 3% Evans Blue dissolved in 0.9% saline for injection. - Directions: remove evans blue stock bottle from 4C and place on magnetic stirrer for 1- 2 hours. Remove 3-4 ml and place in plastic tissue specimen container. Sonicate on setting
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