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- 2026年07月14日
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文献和实验dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
Purpose Working Medium 2) Cell viability and counting a. Pipet 250 μL of a 0.05% Trypan blue solution into one, 1 mL polystyrene round-bottom tube. b. Homogenize the HepaRG™ cell suspension with gentle manual swirling
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
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