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- 2026年07月14日
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4°C. Do not freeze.
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25mL///100mL
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文献和实验with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
of each of the appropriate primer at 5μM working stock concentration Assemble reactions on ice. Incubate in Thermacycler: a) Initial denaturation: 94°C for 4 min b) 30 cycles: Denature at 94°C for 30 sec Anneal at 55°C for 20�30 sec** Extend at 72°C for 45 sec
Methods for the Detection of D-Amino-Acid Oxidase
were mixed with an equal volume of the sample buffer (63 mM Tris-HCl, pH 6.8, 2% SDS, 5% 2-mercaptoethanol, 10% glycerol and 0.002% bromophenol blue) and were heated in a boiling water for 3 min. The samples (5~50 μg protein per lane) were electrophoresed
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