DiagPoly™ Blue Colored Polyeth

Detection and Analysis of Protein‐Protein Interactions of Organellar and Prokaryotic Proteomes by Blue Native and Colorless Native Gel Electrophoresis

?wide scale in a single experiment. The protocols described in this unit are based on separation of protein complexes by blue native polyacrylamide electrophoresis (BN?PAGE), the most versatile native gel system, and the closely related milder

DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE

greenish-blue, but increases background, making the staining more equivocal. Other modified indolyl-based compounds can be used if alternative colored precipitates are desired. Salmon-gal (Biosynth International) results in a light orange/pink precipitate

组织中ß牛乳糖和碱性磷酸酯酶活性检测

, all of which have galactose linked through a ß-D-glycosidic linkage to a moiety whose properties change upon liberation from galactose (Wallenfels and Weil, 1972). Several substrates yield colored or fluorescent soluble products which are useful when quantifying ß-gal