Exsomes Biotin Labeling & Puri

Biotin and streptavidin have very high affinity capacity for each other, the reaction is highly specialized, the dissociation constant of the formed complex is very small, it is irreversibly reactive, and the stability of the product is high, and the biotin-affinity system has become a general and universal research method in the biological field nowadays. In this kit, biotin is directly coupled to the exosome surface by covalent bonding through chemical linkage, and then the free biotin is removed by SEC molecular exclusion column to obtain a large amount of pure biotin-modified exosomes. The biotin-modified exosomes can be linked to any biotin-labeled protein, peptide or small molecule via streptavidin, realizing the surface modification of exosomal protein, peptide and small molecule. This kit has high efficiency of biotin modification, mild reaction conditions, which can occur under physiological conditions, good reaction specificity, no harmful by-products, no damage to the membrane structure of the exosome, and does not affect the biological activity and function of the exosome, which can realize the large-scale preparation of biotin-modified exosomes.