Absolute Mag™ Ni-TED Magnetic
Agarose Particles His Tagged Protein Purification Kit, 30-150 μm- 询价
- 北京安必奇
- WHM-24BY19
- 2026年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 应用：
His tagged protein purification, IP, Co-IP
- 规格：
10mL
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文献和实验Purification of 6xHis epitope tagged proteins by Ni-NTA-Agarose His标签蛋白纯化
crude sonicated sample Dispense into 1.5 ml eppendorf tubes,spin 14k rpm,4℃,30 min Combine soluble fractions into 1 tube Take 30μl soluble fraction as post-sonication soluble sample Test solubility of target protein by SDS-PAGE/Coomassie Blue Stain
Purification and Characterization of His-Tagged Antibody Fragments
must be applied in order to recover the functional hetero-dimeric protein. Here, we describe a protocol that enables the production and purification of His-tagged antibody fragments from bacterial cell lysates using IMAC, as well as their analysis in ELISA
Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)
blood sample into Processing Plate (supplied) followed by adding 10 ul Proteinase K solution and 10 ul Mag-Bind™ Particle R. Note: Proteinase K and Mag-Bind™ Particles R can be prepared as master mix and add to each at one time. For 96 samples, mix
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