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- 文献和实验
- 技术资料
- 库存:
In Stock
- 检测范围:
0.156-10 ng/mL
- 检测方法:
Sandwich ELISA
- 应用:
Quantitative
- 样本:
serum, plasma, cell culture supernatants, tissue homogenate
- 灵敏度:
0.094 ng/mL
- 规格:
96T
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
with 1x PBS containing 0.01% tween. 9) Add peroxidase conjugate (50ul/well) diluted 1:1000 with 1% BSA (diluted in washing solution as in 8)) and incubate for 1hr @ 37°C. N.B.: Use PRAM (Peroxidase-conjugated Rabbit Anti-Mouse) for Ab raised in mice
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