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- 文献和实验
- 技术资料
- 库存:
In Stock
- 检测方法:
sELISA
- 应用:
Qualitative
- 适应物种:
Human
- 样本:
Feces
- 规格:
96T
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文献和实验Molecular Methods to Study Transcriptional Regulation of Clostridium difficile Toxin Genes
Toxin A (TcdA) and Toxin B (TcdB) are the major virulence factors that contribute to the pathogenesis of Clostridium difficile -associated diarrhoea (CDAD). These enterotoxins act by glucosylation of members of the Rho protein family of small
A simple workflow allows the purification of high-quality DNA and RNA from the same sample (see flowchart). The 96-well purification plates of the kit are rapidly and conveniently processed using either a centrifuge (Centrifuge 4-15C and Plate Rotor 2 x 96
A/B/C Random Amplification Protocol
the Round B PCR program and place the samples on the machine when it reaches 94˚C. Part B -- PCR machine parameters: (94˚C 3’) x1 (94˚C 30”, 40˚C 30”, 50˚C 30”, 72˚C 1’) x35 cycles (4ºC hold) x1 3. To check if the PCR reaction went
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