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- 文献和实验
- 技术资料
- 靶点:
FCV
- 应用范围:
Neut, ELISA
- 适应物种:
FCV
- 克隆性:
1D7
- 保存条件:
Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- 形态:
Liquid
- 亚型:
IgG
- 免疫原:
The original antibody was raised by immunizing BALB/c mice with feline calcivirus infected CRFK cells. After two weeks, mouse lymphocytes and mouse myeloma cells (P3U1) were cell-fused with polyethylene glycol to prepare hybridomas.
- 规格:
200μg///1mg
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
suspension. 5) Label another sample tube for each fluorochrome-conjugated antibody you are using, and add 1 drop of AbC™ capture beads (Component A in the AbC™ Anti-Mouse Bead Kit) to each tube. 6) Add a pre-titrated
Monoclonal Antibody Production
Monoclonal Antibody Production Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform
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