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❏ 产品描述
品名:(±)11(12)-EET-d11 methyl ester
SKU:EK20144341
化学名:rel-(11S,12R)-epoxy-5Z,8Z,14Z-eicosatrienoic acid-d11, methyl ester
化学式:C21H23D11O3
分子量:345.6
外观:see COA
纯度:see COA
储存温度:-20℃
For Research Use Only!
To get more information, please contact us freely.
郑重声明:仅用于科学研究,不可用于诊断或治疗。
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文献和实验Labeling Tubulin with Tetramethylrhodamine Succinimidyl Ester
for 20 min.Chill down the rotor afterwards. 10.Rinse pellet quickly with 2x2 ml warm PEM buffer.Resuspend pellet in 2 ml of cold PEM buffer (solution appears opaque). 11.Incubate at 0℃ for 10 min. 12 Centrifuge in a SS34 rotor at 18,000 rpm,4℃ for 20
Methyl DNA Immunoprecipitation
method does require relatively high methyl-CpG density (11 ). Methylated DNA enrichment by binding to MBDs can, therefore, be sequence dependent and can also show low specificity and potential for false-positive results due to capture of unmethylated DNA
LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
. 9.Resuspend pellet in 1.0 ml of buffer 3.Measure the total volume. 10.Add slowly an equal volume of buffer 4 while stirring.Stir gently on ice for 30 min. 11.Centrifuge in a SS34 rotor at 18,000 rpm,4℃ for 20 min. 12.Run supernatant through the G-25 column
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