Fmoc-O-tert-butyl-L-β-homohydr

Synthesis of Peptide‐Oligonucleotide Conjugates by Diels‐Alder Cycloaddition in Water

) N α ‐(9‐Fluorenylmethoxycarbonyl)‐protected amino acids (Fmoc‐aa‐OH; Novabiochem, Bachem): e.g., for sequences illustrated here, Arg(Pbf), Gly, Leu, Phe, Ser(t Bu), and Tyr(t Bu) (t Bu: tert ‐butyl; Pbf

Disulfide Conjugation of Peptides to Oligonucleotides and Their Analogs

5 ), oligonucleotide synthesis ( appendix 3C ), and amino acid analysis (optional; see unit 4.22 ) NOTE: Amino acid protecting groups are t Bu, tert ‐butyl; Boc, tert ‐butyloxycarbonyl; Fmoc, 9‐fluorenylmethoxycarbonyl

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

) N 2 ‐Isobutyryl‐5′‐O ‐(4,4′‐dimethoxytrityl)‐2′‐O ‐propargyl‐3′‐O ‐[(N,N ‐diisopropylamino) 2‐cyanoethyloxyphosphinyl]guanosine ( 3 , ChemGenes) 5.5 M tert ‐butyl