5-Bromo-1,6-dimethyl-1H-indazo

Western Blots

M Tris pH 9.6 200 µl 1M MgCl2 500 µl 5 mg/ml BCIP in dimethyl formamide (BCIP= 5-bromo-4- chloro-3-indoyl phosphate, toluidine salt), US Biochemical). Store this stock at -20° wrapped in foil; it's light sensitive. 2.5 ml 2 mg/ml NBT (p-nitro blue

Measurement of Cation Movement in Primary Cultures Using Fluorescent Dyes

(ionomycin or 4bromo‐A23187; Molecular Probes) in dimethyl sulfoxide (DMSO) 140 mM CaCl 2 in H 2 O 35‐mm tissue culture disk

Synthesis and Properties of 7‐Substituted 7‐Deazapurine (Pyrrolo[2,3‐d]pyrimidine) 2′‐Deoxyribonucleosides

2: Preparation of 7‐IODO‐7‐Deazapurine Nucleosides Related to 2′‐Deoxyguanosine and 2′‐Deoxyxanthosine Basic Protocol 3: Preparation of 7‐Bromo‐7‐Deaza‐2′‐Deoxyisoguanosine Basic Protocol 4: Determination of pKa Values