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细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1(K214R,E356D,M358L) - Kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized antibodies are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Homo sapiens
- 应用范围:
ELISA
- 浓度:
详见COA
- 靶点:
IL15
- 抗体英文名:
Anti-IL15(ordesekimab biosimilar) mAb
- 抗体名:
Anti-IL15(ordesekimab biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | IL15 |
| Uniprot ID | P40933 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | HuMax-IL15, AMG 714, AMG-714, HuMax-IL-15, MAb146B7, PRV-015 |
| 货期 | In Stock |
| 产品英文名称 | Anti-IL15(ordesekimab biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized antibodies are shipped at ambient temperature. |
| 别名 | IL-15 |
| 应用 | ELISA |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Homo sapiens |
| IgG亚型 | Human IgG1(K214R,E356D,M358L) - Kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000 |
| 应用范围 | Research use only |

Figure 1. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL15 Protein, hFc Tag (PME100882) can bind Anti-IL15(ordesekimab biosimilar) mAb (BME100253) in a linear range of 3.20–400 ng/mL. In order to specifically detect BME100253, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL15RA & IL15 Protein, hFc Tag (N120D) (PME101891) can bind Anti-IL15(ordesekimab biosimilar) mAb (BME100253) in a linear range of 1.6–8 ng/mL.In order to specifically detect BME100253, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL15RA and IL15 Protein, mFc Tag (PME101926) can bind Anti-IL15(ordesekimab biosimilar) mAb (BME100253) in a linear range of 1.6-8.0 ng/mL.
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文献和实验活性既不需要抗原致敏,也不需要抗体参与,且无 MHC 限制,因此 NK 细胞具有「最有效的广谱抗癌细胞」的称号。除了强大的杀伤功能外,NK 细胞还具有很强的免疫调节功能,与机体其他多种免疫细胞相互作用来调节机体的免疫状态和免疫功能。 NK 细胞相关治疗策略的研究中以 CAR-NK 最为火热,NK 细胞经改造后的 CAR-NK 可以精准杀瘤,疗效显著。基于 NK 细胞的 ADCC 抗体药物研发也是开展的如火如荼。此外,自带「免疫记忆」的 NK 细胞在肿瘤疫苗等领域的研究中也被寄予厚望。 图 1. IL15
有氧运动真的能抗癌!Cancer Cell:一周 5 次、每次 30 分钟,就可助力免疫系统杀死癌细胞
发表题为 Exercise-induced engagement of the IL-15/IL15-Rα axis promotes anti-tumor immunity in pancreatic cancer 的文章,研究发现本是针对细菌性侵入的哺乳动物免疫系统竟可以识别癌症细胞,而运动诱导的肾上腺素分泌上升调节对白介素 15(IL-15)有响应的免疫细胞,从而延缓胰腺癌在小鼠上的增殖。IL-15 超级激动剂 NIZ985 可模拟运动产生的有益效果,使「癌王」对免疫治疗敏感。 图片来源
基础。 传统的NK细胞扩增技术是在NK细胞培养体系中添加IL2、IL15等细胞因子以促进NK细胞增殖,但由于扩增效率不高且纯度不够未能得到广泛应用。近年来,有人采用基因工程的方法在K562细胞上同时表达IL-15、CD137L 等分子, 并以此细胞来刺激NK细胞的增殖。IL-15和CD137L联合刺激是PBMC 中NK细胞特异性扩增的基础:作为膜蛋白,通过细胞间的接触刺激,用以诱导NK细胞扩增。IL15能够促进NK细胞的分化成熟,而IL21能够联合IL15分子促进NK细胞的分化成熟,所以本研究在稳定
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