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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1 - kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Biotinylated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Humanized
- 应用范围:
ELISA
- 浓度:
详见COA
- 靶点:
B7-H3
- 抗体英文名:
Biotinylated Anti-B7-H3 (enoblituzumab biosimilar) mAb
- 抗体名:
Biotinylated Anti-B7-H3 (enoblituzumab biosimilar) mAb
- 规格:
100μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | B7-H3 |
| Uniprot ID | Q5ZPR3 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | MG-A271, MGA271 |
| 货期 | In Stock |
| 产品英文名称 | Biotinylated Anti-B7-H3 (enoblituzumab biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | B7-H3;CD276;B7 homolog 3 |
| 应用 | ELISA |
| 有无偶联 | Biotinylated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Humanized |
| IgG亚型 | Human IgG1 - kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000 |
| 应用范围 | Research use only |

Figure 1. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human B7-H3 Protein, mFc-His Tag (PME100012) can bind Biotinylated Anti-B7-H3 (enoblituzumab biosimilar) mAb (BME100010B) in a linear range of 40–200 ng/mL.
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状态和疾病进展的重要指标。 检测原理 Elabscience未包被人B-淋巴细胞激活抗原B7-1(LAB7-1)elisa试剂盒采用双抗体夹心ELISA法,原理清晰、操作可靠 包被:使用试剂盒提供的捕获抗体预包被酶标板。 结合:加入样本或标准品,其中的LAB7-1与包被抗体结合。 检测:依次加入生物素化检测抗体和HRP标记的亲和素,形成“捕获抗体-LAB7-1-检测抗体-亲和素”复合物。 显色:加入TMB底物,HRP催化显蓝色,加终止液后变黄。 定量:在450 nm波长读取OD值
人B7-H4 ( B7-H4 )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 B7-H4 单抗包被于酶标板上,标准品和样品中的 B7-H4与单抗结合,加入生物素化的抗人 B7-H4 ,形成免疫复合物连接在板上,辣根过氧化物酶标记的 Streptavidin 与生物素结合,加入底物工作液显蓝色,最后加终止
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