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AJE 润色服务限时 8 折起,权威机构信任 15+ 年Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1(L234A,L235A,G237A) - Kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized antibodies are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Humanized
- 应用范围:
ELISA, Flow Cyt
- 浓度:
详见COA
- 靶点:
TNFSF15
- 抗体英文名:
Anti-TNFSF15(PF-06480605 biosimilar) mAb
- 抗体名:
Anti-TNFSF15(PF-06480605 biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | TNFSF15 |
| Uniprot ID | O95150 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | PF 6480605,PF-6480605,RVT-3101,1D1-1.31,afimkibart |
| 货期 | In Stock |
| 产品英文名称 | Anti-TNFSF15(PF-06480605 biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized antibodies are shipped at ambient temperature. |
| 别名 | TL1;VEGI |
| 应用 | ELISA, Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Humanized |
| IgG亚型 | Human IgG1(L234A,L235A,G237A) - Kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000, Flow Cyt 1:100 |
| 应用范围 | Research use only |

Figure 1. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFSF15 Protein, hFc Tag (PME100752) can bind Anti-TNFSF15(PF-06480605 biosimilar) mAb (BME100222) in a linear range of 0.64–80 ng/mL. In order to specifically detect BME100222, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 2. Flow cytometry analysis with 15μg/mL Anti-TNFSF15(PF-06480605 biosimilar) mAb (BME100222) on HEK293 cells transfected with Human TNFSF15 protein (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Figure 3. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFSF15 Trimer, hFc Tag (PME101840) can bind Anti-TNFSF15(PF-06480605 biosimilar) mAb (BME100222) in a linear range of 1.6–40 ng/mL. In order to specifically detect BME100222, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 4. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFSF15, His Tag (PME101723) can bind Anti-TNFSF15(PF-06480605 biosimilar) mAb (BME100222) in a linear range of 1.6–40 ng/mL.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠血小板因子 -4(PF-4)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 Platelet Factor 4 单抗包被于酶标板上,标准品和样品中的 PF-4 与单抗结合,加入生物素化的抗大鼠 PF
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β-血小板球蛋白(β-TG)和血小板第四因子(PF4)是血小板α颗粒中特有的蛋白。当体内有过多的血小板被激活,释放反应亢进时,二者血浆中的浓度升高,医学教育网搜|索整理因此,β-TG和PF4是血小板活化的重要指标。二者的检测多采用ELISA法。用抗β-TG或抗PF4的抗体包被微孔板,加入待测血浆,血浆中的或与包被的抗体结合,再加入酶标的抗β-TG抗体或抗PF4抗体,与在板上的β-TG或PF4结合,最后加入底物显色。显色的深浅与受检血浆中β-TG或PF4的含量成正比,从标准曲线中计算出受检血浆
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