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- 文献和实验
- 技术资料
- 免疫原:
inquire
- 亚型:
Human IgG1 - kappa
- 形态:
Powder
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 克隆性:
Monoclonal
- 标记物:
Unconjugated
- 适应物种:
Human
- 级别:
Research use only
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 宿主:
Homo sapiens
- 应用范围:
ELISA; Flow Cyt
- 浓度:
详见COA
- 靶点:
B7-H5
- 抗体英文名:
Anti-B7-H5(onvatilimab biosimilar) mAb
- 抗体名:
Anti-B7-H5(onvatilimab biosimilar) mAb
- 规格:
50μg
| 产品类型 | Biosimilar reference antibodies |
|---|---|
| 靶点 | B7-H5 |
| Uniprot ID | Q9H7M9 |
| 背景 | Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals. |
| 通用名 | JNJ-61610588 |
| 货期 | In Stock |
| 产品英文名称 | Anti-B7-H5(onvatilimab biosimilar) mAb |
| 内毒素 | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| 保质期 | 12 months from date of dispatch |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | VISTA;VSIR;Sisp-1;C10orf54;DD1alpha;Dies1;GI24;PD-1H;PP2135 |
| 应用 | ELISA; Flow Cyt |
| 有无偶联 | Unconjugated |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 宿主种属 | Homo sapiens |
| IgG亚型 | Human IgG1 - kappa |
| 反应种属 | Human |
| 推荐稀释比 | ELISA 1:5000-10000; Flow Cyt 1:100 |
| 应用范围 | Research use only |

Figure 1. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human B7-H5 Protein, hFc Tag (PME101041) can bind Anti-B7-H5 Neutralizing antibody (BME100109) in a linear range of 2.44–625.00 ng/mL. In order to specifically detect BME100109, mouse anti-human Fab-specific antibody was used as detection antibody.

Figure 2. Flow cytometry analysis with 1 μg/mL Anti-B7-H5 (onvatilimab biosimilar) mAb (BME100109) on HEK293 cells transfected with Human B7-H5 protein (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Figure 3. Flow cytometry analysis of antigen binding of anti-human B7-H5 mAb(BME100109). (A) BME100109 does not bind to 293T cells that do not express B7-H5. (B) A clear peak shift of BME100109 was seen compared to the control when incubated with B7-H5-expressing THP-1 cells, indicating strong binding of BME100109 to B7-H5. Antibodies were incubated at 5 μg/mL.
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