ROR1 (zilovertamab)参照抗体

产品类型	Biosimilar reference antibodies
靶点	ROR1
Uniprot ID	Q01973
背景	Research grade biosimilar. Not for use in therapeutic or diagnostic procedures for humans or animals.
通用名	UC-961,cirmtuzumab
货期	In Stock
产品英文名称	Anti-ROR1 (zilovertamab biosimilar) mAb
内毒素	Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization.
保质期	12 months from date of dispatch
无菌性	Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use.
储存与运输	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
别名	ROR1;NTRKR1
应用	ELISA; Flow Cyt
有无偶联	Unconjugated
组分	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
宿主种属	Humanized
IgG亚型	Human IgG1 - kappa
反应种属	Human
推荐稀释比	ELISA 1:5000-10000; Flow Cyt 1:100
应用范围	Research use only

Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human ROR1, His tagged protein PME100399 can bind Anti-ROR1 Neutralizing antibody  (BME100073) in a linear range of 0.64-16 μg/ml.

Figure 2. Flow cytometry analysis with Anti-ROR1 (zilovertamab biosimilar) mAb 15 μg/mL on HEK293 cells transfected with Human ROR1 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).

Figure 3. Flow cytometry analysis of antigen binding of anti-human ROR1 mAb(BME100073). (A) A clear peak shift of BME100073 was seen compared to the control when incubated with ROR1-expressing 8226 cells, indicating strong binding of BME100073 to ROR1. Antibodies were incubated at 2 μg/mL.

Figure 4. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human ROR1(30-164) Protein, hFc Tag (PME101581) can bind Anti-ROR1 (zilovertamab biosimilar) mAb (BME100073) in a linear range of 40–200 ng/mL. In order to specifically detect BME100073, mouse anti-human Fab-specific antibody was used as detection antibody.