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- 文献和实验
- 技术资料
- 保存条件:
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- 保质期:
1year
- 英文名:
Human YAP1 Recombinant Protein (N-GST)
- 库存:
现货
- 供应商:
普健生物(武汉)科技有限公司
- 规格:
50ug/100ug/1mg
| 规格: | 50ug | 产品价格: | ¥1268.0 |
|---|---|---|---|
| 规格: | 100ug | 产品价格: | ¥1868.0 |
| 规格: | 1mg | 产品价格: | ¥9764.0 |

| 产品名称 | Human YAP1 Recombinant Protein (N-GST) |
|---|---|
| 货号 | HW509022 |
| 种属 | Homo sapiens (Human) |
| 状态 | Lyophilized |
| 保存溶液 | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| 纯度 | >90% as determined by SDS-PAGE. |
| 应用 | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 内毒素水平 | Please contact with the lab for this information. |
| 表达系统 | E. coli |
| Accession号 | P46937 |
| 蛋白长度 | Ala47-Pro101 |
| 性质 | Recombinant |
| 预测分子量 | 33.77 kDa |
| 重悬 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 运输 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 稳定性和储存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
abinScience, founded in 2023 in Strasbourg, France, is committed to developing and producing high-quality life science reagents. Rooted in one of Europe’s leading hubs for scientific innovation, abinScience empowers global research through reliable, efficient experimental solutions. Guided by the vision of “Empowering Bioscience Discovery,” we support scientists worldwide in advancing the frontiers of bioscience.
Technology and Innovation
Relying on the global leading position in antibody and protein research and more than 20 years of industry experience of its parent company ProteoGenix since 2003, abinScience has inherited advanced technologies and high-quality standards. In particular, the XtenCHO™ system developed by ProteoGenix, which has two modes of transient expression and stable expression, has been widely recognized as a core technology to improve the efficiency of protein production, providing a solid foundation for abinScience’s product line.
Product categories and application fields.
Main products.
10000+ high-activity proteins.
15000+ antibody series products:
Classic monoclonal antibody.
Monoclonal antibody
Internal control antibody, labeled antibody.
High-quality phosphorylation antibodies.
Highly specific nanobodies and small molecule antibodies.
Functional antibodies in vivo.
Low-background flow cytometry antibodies.
DNA/RNA antibodies and PEG antibodies.
1000+ reagent kit series products.
Large-scale packaging customization services: We offer personalized antibody design, protein production, molecular optimization, and experimental development services.
Application field
Infectious disease research: including viruses, superbugs, parasites, etc.
Neuroscience research: Supporting research on neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease.
Immunology research: Covers the research needs of immune activation, inhibition, and allergies and abnormal reactions.
Oncology research: Supporting the development of tumor biology and anti-tumor drugs.
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文献和实验Preparation of N-GST Fusion Protein for Affinity Immobilization of RNA
in a few hours under non-denaturing conditions. However, the performance of affinity purification methods can vary tremendously depending on the RNA immobilization matrix. It was previously shown that RNA immobilization via an optimized λN-GST fusion protein
植物源重组人胰岛素生长因子融合蛋白(OsrIGF-1),胰岛素在无血清细胞培养中的最佳替代品。 IGFs家族由两种低分子多肽(IGF-Ⅰ、IGF-Ⅱ)、两类特异性受体及六种结合蛋白组成。IGF-Ⅰ是一个有70个氨基酸的单链碱性蛋白,分子量7649Da,耐热。IGFs的生物学功能不只局限于有丝分裂刺激作用,它们也能诱导分化或促进分化功能的表达。全新推出的植物源重组人类胰岛素生长因子-1融合蛋白(OsrIGF-1)在IGF-1的N末端融合了人血清白蛋白(HSA),HSA作为蛋白质保护剂增加了IGF
一、GST pull-down 基本知识1. GST pull-down 概念GST pull-down:是利用重组技术将探针蛋白与 GST 谷胱苷肽巯基转移酶融合,融合蛋白通过 GST 与固相化在球珠上的 GSH 谷胱甘肽结合。从而分离出能与融合蛋白相互作用的蛋白的技术。2. GST pull-down 研究对象二、GST pull-down 原理1. GST pull-down 结合原理2. GST pull-down 分离原理三、GST pull-down 流程1. 蛋白的抽提与纯化:①
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