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文献和实验7b. Pool the supernatants from step 6 into 500 ml bottles and add DNase-free RNase A and RNase T1 such that the final concentration of RNase A is 40 ug/ml and RNase T1 is 40 U/ml. Incubate in a 37degC water bath for 30 minutes. 8b. Add an equal
stirrer Microcentrifuge tubes (1.5-mL, RNase-free) Micropipette and tips (including 1-mL tips) Mortar and pestle (prechilled) PCR tubes (0.2-mL or 0.5-mL, RNase-free) Pipette (glass, drawn out) Rotor for centrifuging 15-mL
RNA purification using TRizol--TRIZOL法提取RNA
to a fresh tube. b.Add 0.5 ml of isopropyl alcohol to precipitate RNA. If this RNA will be used for RT-PCR, first add 50 μl isopropyl alcohol, mix, incubate the samples at room temperature for 5 minutes and centrifuge at 12,000 x g for 10 minutes at 4oC
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