384-well qPCR Plate, 40 μL, Sk
irted, White Frame with White Tube, Cut Corners A24 & P24{40μL 384孔qPCR板，白色框白色管，全裙边，A24&P24双切角}- ￥320 - 1600
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- 2026年07月14日
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| 规格： | 10 pcs | 产品价格： | ￥320.0 |
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文献和实验free shotgun sequencing library from miniprep BAC DNA
with shaking at 225 rpm. Plate 100 μl solution on an Ampicilin-selective LB agar plates containing X-Gal ( 40 μl of 20 mg/ml in dimethyformamide per plate) and IPTG ( 4 μl of 200 mg/ml in ddH2O per plate) for blue/white screening. Grow plates overnight at 37℃
freeshotgun sequencing library from miniprep BAC DNA
, immediately add 1 ml of room temperature SOC medium and transfer the solution from cuvette into a culture tube, incubate at 37℃ for I hr with shaking at 225 rpm. Plate 100 μl solution on an Ampicilin-selective LB agar plates containing X-Gal ( 40 μl
the melted agarose up/down to get a uniform mixture. Total volume of the gel slice should be ~40 µl. Can freeze the gel slice to store. Ligation Add 2 µl 10X ligase buffer to a tube (the commercial NEB stuff is fine). Add 2 µl water. Melt your gel slices
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