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- ABclonal
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- 2026年07月14日
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文献和实验E-Z 96 X-press Plasmid Vacuum Protocol
Note: Do not use biomass large than 3.0. For example: If the OD600 of the culture is 2.0, use a 1.5 ml bacterial culture. 3. Discard medium and remove any remaining liquid in the tube by using a pipettor or inverting the tube on an absorbent paper
or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
Transduction Protocol for Adherent Cell Types
- The starting number of cells may be varied to account for total cell availability. Successful transductions have been carried with as little as 2.0 x 105 cells per well. - The duration the viral supernatant is on the cells can go from 4 hours to 2 days
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