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文献和实验Preparation of Carboxypeptidase Y and Its Properties
the basic liquid, wash it to pH7.0 with distilled water; Soak the resin with 0.5mol/L hydrogen chloride for 30mins, and then suction the solution and wash it to pH7.0 with distilled water; Soak the resin with 0.5mol/L sodium hydroxide solution for 30～60
E.Z.N.A.TM Fast Protocol For FFPE Tissue
must be brought to room temperature before use. 13. Using the same 2 ml collection tube, wash the column with a second 500 ul of RWB Wash Buffer. Centrifuge at maximum speed (10,000 x g) for 2 min to dry the column. This step is crucial for ensuring optimal
6xHis-tagged protein purification using Qiagen Ni-NTA Column
Buffers:Lysis Buffer (1 liter)50 mM NaH2PO4 6.90g NaH2PO4.H2O (MW 137.99g/mol )300 mM NaCl(up to 2M)17.54gNaCl(MW 58.44)or 60ml 5M10 mM Imidazole (up to 100mM)0.68g (MW 68.08 )10 mM BME (up to 20 mM)0.69 ml stock (14.4M )Adust pH to 8.0 using
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